Over the past few decades, the ectoparasitic mite Varroa destructor has been a significant threat to managed honey bee (Apis mellifera) colonies worldwide. Many control methods, including application of synthetic acaricides, have been adopted to control the infestation of varroa mites in honey bee colonies. Synthetic acaricides such as coumaphos and fluvalinate are only effective in reducing susceptible mites. Besides, synthetic acaricides pose multiple threats to honey bee colonies and the environment, necessitating their alteration with non-synthetic options. Naturally occurring compounds are considered an essential alternative control measure for varroa mites. Natural acaricides are derived from plants that contain essential oils or organic acids. The current study investigated the efficacy of formic acid, oxalic acid, and thymol in the control of Varroa mites. These soft acaricides were applied at various concentrations/quantities. Formic acid, oxalic acid, and thymol were all effective at lowering mite population levels. Formic acid, oxalic acid, and thymol can be used in an integrated management plan to control varroa mite populations. This scientific-based information can be shared with the beekeeping community of Pakistan and elsewhere, which will be helpful in managing this parasite that often affects honey bee productivity.